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Lab methods

An engaging and informative illustration of various laboratory techniques, including PCR, Western blotting, and gel electrophoresis, with colorful visuals and elements that represent scientific research.

Lab Methods Mastery Quiz

Test your knowledge of laboratory methods with our engaging quiz! This quiz features a range of questions on techniques like PCR, gel electrophoresis, and more.

Whether you're a student, teacher, or research enthusiast, you'll find this quiz helpful in understanding key lab concepts. Prepare to challenge yourself with:

Multiple choice questions
Technical terminology
Real-world applications

13 Questions3 MinutesCreated by AnalyzingData123

Which of the following statements about polymerase chain reaction (PCR) is FALSE?

PCR uses a DNA polymerase from a thermophilic bacterium.

PCR is particularly powerful because after each cycle of replication, there is a linear increase in the amount of DNA available.

For PCR, every round of replication is preceded by the denaturation of the double-stranded DNA molecules.

Which of the following techniques is not appropriate if you want to examine whether a gene is expressed in a specific tissue?

RNA-Seq

In situ hybridization

Immunohistochemistry

Microarray

Gel electrophoresis separates proteins on the basis of which two factor? (All correct answers are required for the points)

The protein's cell of origin

The protein's size

The protein's net charge

The protein's affinity for a ligand molecule

Which of the following terms describes a mouse in which a particular gene has been genetically modified?

Transgenic mouse

Replicated mouse

Transfected mouse

Hybrid mouse

Analyzing the expression of thousands of genes simultaneously can be done using which of the following techniques? (multiple alternatives)

ELISA

Northern blot

DNA microarrays

Next-generation sequencing (RNASeq)

Western blot

A microtome is used to

Slice thin sections (paraffin-embedded) of specimens

View microscopic organisms

Dissect cellular organelles

Focus short wavelengths of light

Which method is used to study detect the presence of proteins in a sample?

Next generation sequencing

Quantitative-PCR

Southern blotting

Western blotting

PCR uses a DNA polymerase from a thermophilic bacterium.

True

False

PCR is particularly powerful because after each cycle of replication, there is a linear increase in the amount of DNA available.

True

False

For PCR, every round of replication is preceded by the denaturation of the double-stranded DNA molecules.

False

True

The PCR will generate a pool of double-stranded DNA molecules, most of which will have DNA from primers at the 5′ ends.

True

False

Flow cytometry is based upon the use of _____________

Immunological techniques

Radioactive elements

Energy content

Heavy isotopes

DNA sequencing refers to the

Technique used to determine the phosphate sequence in a DNA molecular

Technique used to determine the base sequence in a DNA molecule

All of these

Technique used to determine the sugar sequence in a DNA molecule

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