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RNA Isolation Quiz 2 - Concepts

An illustration of a laboratory setting focused on RNA isolation and analysis, featuring various lab equipment such as pipettes, centrifuges, and gel electrophoresis setups, with vibrant colors and a scientific atmosphere.

RNA Isolation Mastery Quiz

Test your knowledge on RNA isolation techniques and concepts with this comprehensive quiz! Designed for molecular biology enthusiasts, this quiz covers essential topics that will enhance your understanding of RNA handling and analysis.

Do you have what it takes to score perfectly? Dive in and challenge yourself with questions covering:

Nucleic acid stability
RNA handling protocols
Ethical lab practices
Critical analysis of techniques

14 Questions4 MinutesCreated by IsolatingMolecule47

Which of the following is the most stable nucleic acid?

Double stranded DNA

Single Stranded RNA

MiRNA

MRNA

Why do we pass samples through QiaShredder columns?

To homogenize cell/tissue lysates - breaking cells apart releasing cellular components

To break down the RNA inside the cells

To separate DNA from RNA

To shred up harmful RNases

What is the role of 70% ethanol in RNA Isolation?

To wash away proteins

To precipitate nucleic acids

To dissolve nucleic acids

To help the sample forget its troubles

What is the role of RW1 in RNA isolation?

It concentrates DNA in the DNA columnns for R&D extractions

It's proprietary information not given to us

It's a wash buffer that removes non-specific biomolecules bound to the silica membrane while leaving RNA bound to the column

None of the above

Does 80% ethanol perform the same function as 70% ethanol?

Yes, it's only 10% difference, duh!

No, it's like RPE an extra wash to wash away residual salts

No, it washes away the sample's pain

Yes, it purifies the RNA

Why do we perform a dry spin after the 80% ethanol addition?

Ethanol interferes with downstream processes and sample purity

We don't

Ethanol degrades RNA

It angers the lab gods

What causes RNA to elute from the column?

EDTA is used to bind to RNA at several points, removing it from the column after spinning.

Water is a polar molecule that can interact electrostatically with nuceleic acids, allowing them to easily dissolve in water to be spun into elution tubes.

An act of God.

None of these.

Which reagents contain guanidine salts?

RW1

RPE

RLT

Both RW1 & RLT

True or False: We should mix reagents that contain guanidine salts with bleach.

True: It inactivates the salts

False: It creates toxic volatile gases

What happens during RNA Quantnorm?

Samples are normalized to 5ng/uL these sample volumes can vary based on the dilutions (C1V1=C2V2)

RNA is amplified

Samples are brought to the same volume

None of these.

What are SpeedVac samples?

Low concentration DNA

Low Concentration RNA

Garbage

RNA at 5ng/uL

What are the 2 peaks that we are looking for when we run Bioanalyzers?

18S and 28S ribosomal subunit peaks

16S and 26S ribosomal subunit peaks

None of these

18S/28S and 16S/26S

What type of cells are our samples from?

Prokaryotes

Eukaryotes

Viruses

Amoebas

What are RINs?

An RNA Integrity Number that is the ratio of 18S:28S subunits

RNA Integration Number that's the ratio of total RNA:mRNA

RNA Intensity Number that tells the sample's A260/A280

None of these.

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