HPCT LEC: TRIMMING AND SECTIONING

Process of removing excess wax after embedding.

Give one other name for sectioning.

Process by which a processed tissue is cut into uniformly thin slices to facilitate studies under the microscope.

SECTIONING

One side of the knife is flat and other other is concave.

Both sides are concave.

Both sides are straight.

Recommended for frozen sections or extremely hard and tough specimens.

Recommended for cutting paraffin embedded sections on a rotary microtome.

Clearance angle

Bevel angle

Cutting angle

Main purpose of honing.

Main purpose of stropping.

For manual sharpening when cutting edge has been rendered blunt or nicked.

Gives the best result.

Much coarser and used only for badly nicked knives.

Gives more polishing effect.

Simplest among the microtomes.

For cutting serial sections of large blocks of paraffin embedded tissues.

Most common type used for both routine and research labs.

For cutting sections for EM.

For cutting unembedded frozen sections.

Microtome used when rapid diagnosis is required.

For cutting frozen sections.

Most commonly used for rapid preparation of urgent tissue biopsies for inoperative diagnosis.

Most dangerous type of microtome.

Recommended for extremely hard and rough tissue blocks.

Originally made for cutting very large blocks such as whole brain.

How long does it take to dry tissue on slides using drying oven?

Size of the knife used in base sledge microtome.

Why is the standard sliding microtome considered the most dangerous type?