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Week 1 Revision

An illustrated diagram of chromatography techniques, featuring colorful protein bands, chemical structures, and laboratory equipment in a science lab setting.

Week 1 Revision Quiz: Chromatography & Protein Analysis

Test your knowledge on chromatography and protein analysis with our comprehensive quiz! This quiz covers essential principles, techniques, and calculations involving protein purification and characterization. Challenge yourself to see how much you remember!

11 engaging questions
Multiple choice, checkbox, and text input formats
Score yourself and improve your understanding

11 Questions3 MinutesCreated by RunningScience25

What is the main principle behind chromatography?

Identifying types of protein

Separating components of a mixture

Solubilising components of a mixture

Determining protein concentration

You have a His tagged protein how will you purify it? (Choose the best option)

Affinity Chromatography with Ni bound resin

Size exclusion based upon weight

Charge separation using pI

What is pI?

Protein of interest

Isoelectric point

PH at which net charge=0

PH at which net charge=1

Select the right statements about pI

When pH=pI Charge of protein=1

When pH=pI Charge of protein=0

When pH>pI Charge of protein is negative

When pH>pI Charge of protein is positive

In size exclusion chromatography:

Larger bands come out first

Smaller bands come out first

Bands come out based on the proteins pI

Bands come out based on the pH of the elution buffer

What is the Beer Lamberts equation. (Note: Re-arranged to find concentration, e is epsilon)

C=A/el

C=Ael

C=el/A

In the Beer Lamberts Law what is the molar extinction coefficient

E (M-1 c-1)

E (M cm-1)

E (M cm)

E (M-1 cm)

You have Purified Protein X. At 1:10 dilution its absorption value at 280nm is 35 and its molar extinction coefficient is 46995 (Use units from previous answer). What is the stock protein concentration?

0.7447mM

0.1644mM

7.447mM

16.44mM

74.47 uM

16.44uM

You have dissolved 10g of NaCl (M.W= 58.44 g/mol) in 100 ml Distilled water (M.W= 18.01g/mol). What is the concentration of the Solution.

5.55 M

0.584 M

0.1801 M

1.711 M

Using the above values, how will you make 1M NaCl solution

58.44g NaCl in 500 ml H20

5.844g NaCl in 1L H20

0.05844 mg in 100 ml H20

58.44 g in 100ml H20

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