Gene Editing Techniques

What is the main purpose of gene editing techniques?

To cure genetic diseases

To create genetically modified organisms

To enhance human intelligence

To clone animals

What is the main advantage of using TALENs over traditional gene editing techniques like ZFNs?

Higher specificity

Lower cost

Faster editing

Greater efficiency

Which of the following is a characteristic feature of homing endonucleases?

They are large enzymes with complex structures.

They exclusively target exogenous DNA.

They have a high mutation rate.

They require a guide RNA for targeting.

What is the role of guide RNA in CRISPR-Cas9 gene editing?

It identifies the target gene

It cuts the DNA

It repairs the edited gene

It delivers the Cas9 protein

Which gene editing technique utilizes a DNA-cutting enzyme called FokI?

TALENs

ZFNs

CRISPR-Cas9

RNA interference

What is the potential ethical concern associated with gene editing techniques?

Off-target effects

Creation of designer babies

Loss of genetic diversity

Increased risk of cancer

Which gene editing technique has been successfully used to treat sickle cell disease?

CRISPR-Cas9

TALENs

ZFNs

RNA interference

Which of the following is a key factor influencing the specificity of the CRISPR-Cas9 system?

Length of the guide RNA (gRNA)

Cas9 protein concentration

Presence of off-target sites

Number of repeats in the CRISPR array

Which gene editing technique is based on the use of transcription activator-like effectors (TALEs)?

CRISPR-Cas9

TALENs

ZFNs

RNA interference

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