HPCT Lec Midterm: Impregnation and Embedding

Simplest, most common, best embedding medium used for routine tissue processing.
Paraffin wax impregnation can be prepared within ________.
Melting point used for routine work.
If the lab temp is 15-18C, the melting point to use should be _______.
This method makes use of an automatic tissue processing machine.
In automatic processing, how many changes of wax are required to remove the clearing agent?
Involves the wax impregnation under negative atmospheric pressure inside an embedding oven.
Provides more rapid wax penetration of tissue.
Mixture of highly purified paraffin and synthetic plastic polymers.
For large dense tissue blocks such as bones and brain.
Less brittle and less compressible than paraplast.
MELTING POINT
Paraplast
56-57C
Embeddol
56C
Ester wax
46-48C
Lab temp=20-24C
54-58C
Lab temp=15-18C
50-54C
Routine work
56-58C
Synthetic wax
A product of paraffin, containing rubber, with the same property as paraplast.
Harder than paraffin; not soluble in water.
Soluble and miscible with water; suitable for many enzyme histochemical studies.
Very slow (days to weeks)
Rarely used impregnating media.
Done after wax impregnation.
Process by which the tissue is arranged in a precise positions in the mold.
Placing the tissue in a mold containing the medium.
Embedding media/method used extensively for light microscopy.
Reduces antigenicity, toxic and damage tissue.
Embedding media/method that is not often used.
Preferred for processing of whole eye sections.
Recommended for bones, teeth, large brain sections, and whole organs.
Another form of celloidin soluble in equal conc of ether and alcohol.
Forms a harder tissue block and makes cutting of thinner sections possible.
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