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DdPCR and QX One Quiz

Generate a detailed illustration of a ddPCR laboratory setup, featuring the QX One device, droplets being analyzed, and scientists discussing results in a modern lab environment.

ddPCR and QX One Quiz

Test your knowledge on digital droplet PCR (ddPCR) and the QX One system with this engaging quiz designed for professionals and enthusiasts alike.

Whether you are a researcher or a laboratory technician, this quiz will help reinforce your understanding of key concepts:

ddPCR methodology
Quantification techniques
QX One cartridge features

10 Questions2 MinutesCreated by TestingGuru42

DdPCR calculates the target concentration using what method?

Standard Curve

Poisson Distribution

Colony Counts

Predictive Analysis

What time of quantification does ddPCR provide?

Qualitative/Semi-Quantitative

Relative Quantitative

Absolute Quantification

What are the 4 channels that can be read on the QX One?

FAM/HEX/ROX/CY3

FAM/HEX/Atto425/CY5

FAM/HEX/CY5/CY5.5

FAM/HEX/TEXASRED/SybrGreen

What is the minimum number of droplets that are required for a well to be considered valid?

1,000

5,000

10,000

20,000

At what stage do the droplets become stable?

Droplet Generation

Droplet Reading

Thermocycling

What volume should each well of the GCR96 cartridge be filled with?

5uL

50uL

20uL

15uL

The GCR96 Cartridge is sealed once in the PX1 Plate Sealer?

True

False

Which setting cannot be changed after the plate has run?

Supermix

Plate Template

Plate name

Experiment Type

Thresholds for positive and negative droplets are typically set using which samples?

Negative Controls

Positive Controls

Test Samples

When setting the Threshold, which tool is used for applying the Threshold to the rest of the plate/assay?

Threshold Single Well

SD Multiple Wells

Threshold Multiple Wells

Auto Threshold

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