SEMEN ANALYSIS
How long does it take for the sperm to mature and develop flagella in the epididymis?
90 days
72 days
3 weeks
7 days
Transport medium for the sperm.
Prostate fluid
Seminal fluid
Alkaline mucus
Metabolized by the spermatozoa for motility.
Fructose
Glucose
Citric acid
Acid phosphatase
Responsible for the gray appearance of semen.
Flavin
Fructose
Acid phosphatase
Alkaline mucus
Responsible for both coagulation and liquefaction of semen after ejaculation.
Seminal fluid
Prostate fluid
Bulbourethral glands
Seminiferous tubules
Neutralize acidity from the prostate secretions and the vagina.
Alkaline mucus
Prostate fluid
Seminal fluid
When the first portion of the ejaculate is missing, the sperm count will be _______, the pH will be falsely _______, and the specimen will ________.
Decreased; increased; not liquefy
Decreased; decreased; not liquefy
Decreased; increased; liquefy
Increased; decreased; liquefy
MATCHING TYPE: Match the structure to its function.
Epididymis
Sperm maturation
Seminal vesicles
Provide nutrients for sperm and fluid
Prostate gland
Provide enzymes and proteins for coagulation and liquefaction
Ductus deferens
Propel sperm to ejaculatory ducts
Bulbourethral glands
Spermatogenesis
Seminiferous tubules
Add alkaline mucus to neutralize prostatic acid and vaginal acidity
Semen composition: Prostate fluid
5%
60-70%
20-30%
Semen composition: Spermatozoa
5%
60-70%
20-30%
Semen composition: Bulbourethral glands
5%
60-70%
20-30%
Semen composition: Seminal fluid
5%
60-70%
20-30%
Urine is toxic to sperm, thus affects the evaluation of sperm motility.
True
False
Normal sperm count.
>40 million/ejaculate
<40 million/ejaculate
>20 million/ejaculate
<20 million/ejaculate
Normal sperm concentration
>40 million/ml
<40 million/ml
>20 million/ml
<20 million/ml
Suppose the specimen has not liquefied after 2 hours, what would you add to induce liquefaction for the specimen to be analyzed?
NSS
Bromelain
NOTA
AOTA
Watery (0) to gel like (4)
Viscosity
Liquefaction
Volume
Color
MATCHING TYPE: Alternative Sperm Motility Grading Criteria
Progressive motility
Sperm moving with an absence of progression
Immotility
No movement
Nonprogressive motility
Sperm moving linearly or in a large circle
Sperm motility is examined:
Within 1 hour of collection
Immediately after collection
After 6 hours of incubation
After 2 hours
MATCHING TYPE: Sperm Motility Grading
Motile without forward progression
4.0
No motile
3.0
Motile with slow forward progression, noticeable lateral movement
1.0
Motile with slower speed, some lateral movement
0
Motile with rapid, straight line motility
2.0
Abnormalities in the following sperm morphology affects motility except:
Neckpiece
Head
Midpiece
Motility
Which sperm morphology produces energy required for motility?
Neckpiece
Head
Midpiece
Tail
Possible abnormality for decreased sperm count.
Vitality
Lack of seminal vesicle support medium
Male antisperm antibodies
Female antisperm antibodies
A single motile sperm on a wet preparation is an indication of an unsuccessful vasectomy.
True
False
Major component of seminal fluid.
Fructose
Glucose
ACP
Citric acid
Failure to document the time of collection primarily affects the interpretation of:
Appearance
Volume
PH
Viscosity
The primary reason to dilute a semen specimen before performing a sperm concentration is to:
Immobilize the sperm
Facilitate the chamber count
Decrease the viscosity
Stain the sperm
The percentage of sperm showing average motility that is considered normal is:
25%
50%
60%
75%
The purpose of the acrosomal cap is to:
Penetrate the ovum
Protect the nucleus
Create energy for tail movement
Protect the neckpiece
What test/s is/are requested for forensic cases?
Acid phosphatase and glycoprotein p30
Acid phosphatase and citric acid
Citric acid
Glycoprotein p30 and citric acid
Failure to collect the _____ portion during ejaculation may give false decreased sperm count.
First
Last
Middle
Failure to collect the ______ portion during ejaculation may alter liquefaction and sperm motility.
First
Middle
Last
Used to test sperm viability.
Eosin-nigrosin stain
Pap stain
Wright stain
Fructose test
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