BIOL 230 Lab Exam 1 Review
BIOL 230 Lab Exam 1 Review Quiz
Test your knowledge and understanding of microbiology concepts with this comprehensive 29-question quiz! Dive deep into bacterial shapes, media types, and key laboratory techniques to prepare for your upcoming lab exam.
- Multiple choice, checkbox, and free response questions
- Covers essential topics in microbiology
- Ideal for students in BIOL 230 and similar courses
Bacillus have what shape?
Rod
Sphere
Spiral
Cube
Can staphylobacilli exist?
NO
YES
Yeast are fungi therefore select all that is true.
Unicellular
Prokaryotic
Eukaryotic
Multi-cellular
Reproduce through Budding
Replicate through binary fission
Spherical
1-5 micrometers
1 milliliter equals how many liters?
10
100
1000
1000000
The approximate size of a microorganism can be determined using the ocular micrometer when using 1000x.
True
False
Selective medium ___________ the growth of unwanted microorganisms.
Enriches
Inhibits
Select all that apply to differential media.
Change in shape of microorganism
Change in size of microorganism
Change in color of mcroorganism
TSA agar plates
CNA agar plates
MAC agar plates
Enrichment media enhances the growth of certain organisms.
True
False
CNA agar plates grows gram negative and MAC agar plates grow gram positives.
True
False
The streak plate method is used to obtain and isolate _______ cultures.
Mixed
Pure
Mac agar plates contains the sugar lactose and is a selective and differential medium therefore what should you expect in the agar?
Fermentation
Gram positive microorganisms
Gram negative microorganisms
Change in color
Are these steps correct if not what are the corrections? Sterilize inoculating loop for 5 sec. -> remove cap of broth culture w/ index and thumb -> Micro-incinerator the opening of tube for 2-3 sec. -> insert inoculum and take sample -> Micro-incinerate tube for 2-3 sec. -> Air dry for 30 sec. -> Cap the tube.
Sterilize inoculum for 10 sec.
Sterilize inoculum for 8 sec.
Remove cap w/ middle and thumb finger.
Remove cap w/ pinky finger.
Air dry for 10 sec.
DO NOT AIR DRY!!!!!!
Serial dilution = series of dilutions come on people!!!!
Serial dilution = series of dilutions come on people!!!!
The instrument used to measure turbidity is a spectrophotometer.
True
False
The percent of light transmitted through the tube is inversely proportional to the bacterial concentration.
True
False
The amount of light absorbed in the tube is directly proportional to the bacterial concentration.
True
False
CFU (Colony Forming Units) = # of colonies x dilution factor / volume plated
True
False
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