Experiments (before Rosen et al’s publication) showing that that PPARγ can induce adipogenesis and cause transdifferentiation show that PPARγ is ____ to induce adipogenesis?

Necessary

Sufficient

Cell Dependent

Cool Enough

This paper wanted to show that PPARγ is ______ to induce adipogenesis?

Necessary

Sufficient

Irrelevant

Acting as a mitogen

Why didn’t the authors just create a homozygous null mutant?

It felt like a lot of work and they didn’t have the time/energy

The confounding effects of PPARγ knockdown meant that the embryos died before forming adipose tissue

Previous efforts had failed to make an actual homozygous null mutant

It had already been done and the authors were using mouse chimeras to confirm the results

What is a mouse chimera?

A mouse with the head of a goat and the tail of a snake

A single organism composed of cells of different genotypes

A single organism composed of tissues made of the same genotype but from different species

A single organism in which the zygote has been targeted via a target vector to change its genotype

In class we’ve talked about different ways to make knockout cells. Which of the following methods did the authors use in this paper to generate their chimera?

A targeting vector that uses homologous recombination through Cre/Lox

CRISPR/Cas9 introduced by a vector

RNAi introduced by a vector

A targeting vector that uses homologous recombination through FRT/FLP sites

Wishful thinking

What are Southern blots used for?

Detection of specific proteins

Detection of specific DNA sequences

Detection of specific mRNA sequences

Finding the nearest chicken and biscuit joint.

Why did the authors look into the gene dosage effect of PPARγ?

With the time they saved on not making a homozygous null mutant, they finally had time for this experiment

It confirms that PPARγ is sufficient for adipogenesis

It confirms the results from Figures 2 and 3 in vitro

It shows if PPARγ is necessary for early adipose cell determination, subsequent lipogenesis or both

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