HPCT MIDTERM: Exam Review Questions

Aim of clearing.

Aim of trimming.

Aim of decalcification.

Aim of dehydration.

Ideal time required for decalcification.

Decalcifying agent recommended for urgent biopsies.

Clearing agent recommended for urgent biopsies.

Recommended for routine decalcification of postmortem research tissues.

Decalcifying agent used for teeth and small pieces of bones.

Decalcifying agent suitable for minute pieces of bones.

Decalcifying agent suitable for minute bone spicules.

Decalcifying agent suitable for small spicules of bones.

DECALCIFYING AGENTS

Increases tissue solubility by hastening decalcification using formic acid that contains decalcifying solution.

Sequestration of calcium

Shorter time for calcium removal.

Which of the following statements is true about standard sliding microtome?

Most ideal and most sensitive method of measuring decalcification rate.

Affects the rate at which calcium is removed.

The larger the specimen, the longer the decalcification time.

High corrosive and carcinogenic decalcifying agent.

A tissue softener that makes tissue appear swollen and soapy.

Softens tissue and allows for easier sectioning.

Tissue to fluid volume ratio.

Dehydrating agent to tissue ratio.

Best dehydrating agent.

Best general decalcifying agent.

Most rapid decalcifying agent.

Excellent dehydrating and clearing agent.

Slow dehydrating agent

Dehydrating agent recommended for delicate tissues such as embryonic and animal tissues.

Toxic fixative and dehydrating agent.

Toxic dehydrating and clearing agent.

Preserves negri bodies.

Clearing agent that causes minimum shrinkage.

Used to dehydrate smears producing minimal shrinkage.

Most commonly used clearing agent.

Serves as an indicator that dehydration is complete.

Substitute for benzene or xylene

Noncarcinogenic clearing agent.

Clearing agent recommended for CNS tissue and cytology.

Similar to chloroform but cheaper.

Clearing agent recommended for very delicate tissues.

No dealcoholization but make the tissue clearer.

Turns milky which indicates incomplete dehydration

Chloroform may cause ___________.

CLEARING TIME

Best embedding medium.

Less brittle and less compressible than paraplast.

Embedding medium used for large dense tissue blocks.

Embedding method that is toxic and damage tissues.

Celloidin method used for whole organ and brain sections.

Celloidin method used for whole eye sections.

A form of celloidin soluble in equal concentration of ether and alcohol.

MELTING POINTS

TYPES OF HONES

What is added to gelatin to prevent molds?

Embedding medium that is soluble and miscible with water.

Used for embedding eye sections.

Process by which a processed tissue is cut into uniformly thin slices to facilitate studies under the microscope.

What is the thickness of the tissue blocks for routine histology?

What is the thickness of the tissue blocks for frozen section?

What is the thickness of the tissue blocks when a cold microtome is used?

What is the thickness of the tissue blocks when a rotary microtome is used?

What is the thickness of the tissue blocks when a rocking microtome is used?

Both sides are concave.

Recommended for cutting paraffin embedded sections on a rotary microtome.

One side of the knife is flat and the other is concave.

Recommended for frozen sections or extremely hard and tough specimens.

Both sides are flat.

How long does it take to dry processed tissue on slides using drying oven?

Which of the following statements is/are true about the purpose of honing?

Which of the following statements is/are true about the purpose of stropping?

Recommended for extremely hard and rough tissue blocks.

Most common type used for both routine and research labs.

Most commonly used for rapid preparation of urgent tissue biopsies for inoperative diagnosis.

Most dangerous type of microtome.

Simplest among the microtomes.

For cutting frozen sections.

For cutting sections for EM.

For cutting serial sections of large blocks of paraffin embedded tissues.

For cutting unembedded frozen sections.

Originally made for cutting very large blocks such as whole brain.