Handling of Biohazards in the Laboratory

Enter your first and last name.

True or false: Acids and bases can be stored together.

True

False

Name four things you must wear when handling cultures and any other biohazard.

Fill in the blank: All used media plates must be _______________ at 121 C for ____________ minutes minimally.

Fill in the blank: The _______________ sheet lists any hazards identified for a product. Review and sign off that each technician understands the potential risks for each chemical/item they use in the lab.

Fill in the blank: Used media plates are bagged to prevent leakage during sterilization and once removed from the autoclave, the biohazard bag is placed into a _________ bag before being disposed in the regular trash.

Fill in the blank: If a calibrated min-max thermometer is not available, a _______________ or calibrated autoclave thermometer (installed in the autoclave) must be used. Document results.

Fill in the blank: Gloves used to handle anything with growth must be ____________ before disposal.

Fill in the blank: Lab coats in the microbiology lab must have ___________ in order to prevent the technician from exposure to biohazards.

Fill in the blank: Caps on bottles must be ___________ before being autoclaved. Containers must be ___________ % full or less to allow for expansion.

Fill in the blank: _____________ must be provided between items being autoclaved to allow for effective sterilization.

Fill in the blank: Autoclaves must be verified ____________ against all safety parameters which includes the ____________ gauge, emergency exhaust, and thermometer.

True or False: The autoclave certificate must include the results of the verification tests as well as any maintenance performed by the third party.

True

False

Fill in the blank: Preventative maintenance performed by the laboratory internally must be ____________ for historical purposes.

For the following image, please match the appropriate pictogram to its meaning. Please answer by placing the corresponding letter in numerical order.

Fill in the blank: The greatest risk of personal injury occurs during the time items are ____________. This is why one must wear the extra PPE of ____________, ____________, and face shield.

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