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Week 2 revision quiz

Create an educational illustration depicting a laboratory setup for protein purification, focusing on KARI-6xHis, with visual elements like a lysozyme bottle, a desalting column, and a BCA assay color change.

Week 2 Revision Quiz: Mastering KARI-6xHis

Test your knowledge on the processes and key concepts related to the purification and analysis of KARI-6xHis. This quiz is designed to reinforce your understanding from Week 2 of our study program. Whether you're prepping for an exam or just looking to refresh your knowledge, this interactive quiz will help solidify your grasp of the material.

10 challenging questions
Multiple-choice format
Enhance your understanding of protein purification

10 Questions2 MinutesCreated by ExtractingEnzyme42

Why do we add Lysozyme?

To digest proteins from the lysate

To break down the cell wall

To digest DNA

To make the cells grow

What does the induced cell lysate contain

KARI only

All proteins produced by the cell

KARI-6xHis only

6xHis only

What is the function of Binding Buffer

To bind the KARI-6xHis to the resin

To clean up the column

To cleave KARI-6xHis

To wash proteins that aren't bound to the resin

What is the function of wash buffer

To bind the KARI to the resin

To clean up the column

To cleave KARI-6His

To wash proteins that aren't bound to the resin

Why does the elution buffer contain the highest concentration of immidazole?

Immidazole binds to KARI-6xHis and precipitates it

Immidazole competitively binds to the resin thereby eluting KARI-6xHis

Immidazol cleaves the bond between KARI-6xHis and elutes only KARI

Immidazol denatures the His tag leading to elution

Immidazole unfolds KARI-6xHis leading to its elution

Why do we do a desalting step of the eluted fractions containing KARI-6xHis?

To get rid of random proteins

To get rid of KARI co factors

To get rid of the His tag

To get rid of any salts from the eluted fractions.

How does the Desalting column (G-25 Sephadex column work)?

Size exclusion (Smallest molecule out first)

Size exclusion (Biggest molecule out first)

Charge based elution (Highest charge out first)

Charge based elution (Lowest charge out first)

What does the BCA assay identify?

Only KARI proteins

Only His tagged proteins

All proteins in the solution

Only presence of salt contamination

BCA creates a colour absorbed at what wavelength?

Purple at 471

Purple at 700

Purple at 562

Blue at 471

Blue at 562

Why do we do a enzymatic reaction of elutions that show the presence of protein

To identify the presence of active KARI

To identify the presence of active all KARI

To identify the presence of proteins in the lysate

To identify the presence of contaminants

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