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Chromatography

Chromatography Challenge

Test your knowledge on the fascinating world of chromatography! This quiz covers essential concepts, techniques, and practical applications in analytical chemistry. Whether you're a student, teacher, or just a curious mind, this quiz is designed to challenge you and enhance your understanding.

Key Features:

Multiple choice questions
Real-world applications
Ideal for students and professionals alike

15 Questions4 MinutesCreated by AnalyzingAtom523

The number of moles of solute present in 1 kg of a solvent is called its

Molality

Molarity

Normality

Formality

Which of the following properties is not used as a basis for physical separation of analyte species in analytical chemistry?

Mass

Polarity

Molar absorptivity

Physical state

Size

What is the main difference between HPLC and UPLC?

HPLC is reverse-phase whereas UPLC is normal-phase

UPLC employs smaller stationary-phase particle size

HPLC and UPLC employ different mobile phases

HPLC and UPLC employ different detection methods

HPLC operates at higher mobile phase pressures

Accuracy is defined as:

A measure of how often an experimental value can be repeated.

The closeness of a measured value to the real value.

The number of significant figures used in a measurement.

None of these

How many significant figures are present in the number 10,450?

Three

Four

Five

None of these

Chromatography is used to:

Separate two or more compounds based on their polarities.

Separate two or more compounds based on their masses.

Separate two or more compounds based on how strongly they interact with other compounds.

All of these

None of the above

If I dilute 5 mL of 0.15 M NaCl to a final volume of 5 L, what’s the final concentration of NaCl?

If it takes 5 mL of 1.4 M NaOH to neutralize 150 mL of HCl with an unknown concentration, what was the original concentration of the acid?

0.467 M

0.047 M

0.014 M

None of these

What is the solid particles used in chromatography called?

The solid phase

The stationary phase

The column phase

The particular phase

What is the liquid used in chromatography called?

The pumped phase

The transparent phase

The mobile phase

The solution phase

What makes the liquid pass through the HPLC column?

The capillary forces

A pump

Gravity

Electricity

What is the function of the injector?

It injects the sample solution into the HPLC system

It injects energy into the column to facilitate the separation

It injects light into the detector

It injects liquid into the reservoir

Why should not mobile phases with pH above 8 be used with silica based columns?

The silica dissolves at high pH so that the column deteriorates

The mobile phase becomes flammable at high pH

The pressure becomes too high

The analytes degrade at high pH

Which organic modifiers are most common in Reversed Phase Chromatography

Isopropanol and toluene

Methylenechloride and chloroform

Methanol and Acetonitrile

Ether and acetone

Chromatographic retention is due to:

Different injection times by the autosampler

Adsorption of the analyte to the stationary phase

Differences in absorbance in the UV detector

Deviations in the flow from the pump

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