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DdPCR and QX One Quiz

A visually appealing illustration depicting the ddPCR process, highlighting droplet digital PCR technology and laboratory equipment related to QX One, in a colorful and educational style.

Master ddPCR and QX One Quiz

Test your knowledge on ddPCR and the QX One system with this engaging quiz designed for researchers, technicians, and students alike. Challenge yourself to understand the intricacies of droplet digital PCR technology.

Key Features:

10 multiple-choice questions
Assess your understanding of ddPCR concepts
Immediate feedback on your answers

10 Questions2 MinutesCreated by ExploringScience42

DdPCR calculates the target concentration using what method?

Predicative Analysis

Colony Counts

Poisson Distribution

Standard Curve

What type of quantification does ddPCR provide?

Relative Quantitative

Qualitative/Semi-Quantitative

Absolute Quantification

What are the 4 channels that can be read on the QX One?

FAM/HEX/TEXASRED/SybrGreen

FAM/HEX/Atto425/CY5

FAM/HEX/ROX/CY3

FAM/HEX/CY5/CY5.5

What is the minimum number of accepted droplets that are required for a well to be considered valid?

20,000

10,000

1,000

5,000

At what stage do the droplets become stable?

Droplet Generation

Thermocycling

Droplet Reading

What volume should each well of the GCR96 cartridge be filled with?

15uL

20uL

50uL

5uL

The GCR96 Cartridge is heat sealed once in the PX1 Plate Sealer?

True

False

Which setting cannot be changed after the plate has run?

Supermix

Plate Template

Experiment Type

Plate Name

Thresholds for positive and negative droplets are typically set using which samples?

Negative Controls

Positive Controls

Test Samples

Standard

When setting the Threshold for your plate/assay, which tool is used?

Threshold Single Well

SD Multiple Wells

Threshold Multiple Wells

Auto Threshold

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