P067 Assay Quiz Part 1

Test your knowledge on the P067 assay and its various components!

This quiz covers crucial aspects of MDR1-MDCK and Caco-2 assays, including controls, inhibitors, and techniques used in the field.

10 Questions2 MinutesCreated by AnalyzingData42

Which of the following controls are used in a standard MDR1-MDCK assay?

Talinolol & Prazosin

Antipyrine & Prazosin

Antipyrine & Propranolol

Atenolol & Talinolol

Which of the following controls are used in a standard Caco-2 assay?

Atropine, E3S, Atenolol & Antipyrine

Prazosin, Propranolol, Atenolol & E3S

Digoxin, Atenolol, Talinolol & Antipyrine

Antipyrine, Atenolol, Talinolol & E3S

Why might we get high or low C0s in our data?

Poor solubility

Poor mixing

Too much/little buffer added

All of the above

Why is it important to calculate recovery of test compound in our assays? Select all that apply.

To determine whether there has been any non-specific binding

For calculating the Papp

To determine if there are potential solubility issues

To ensure the compound has not been retained within the cells

To see if the compound has been metabolised intra-cellularly

To ensure the cells have formed a confluent monolayer

Finish the sentence. Lucifer yellow is used in P067 assays in order to...

Mark which solution is our donor dosing solution

Confirm efflux transporters are functioning correctly

Ensure the cells have formed confluent monolayers

Increase the solubility of test compounds

Antipyrine is our...

High permeability control (97%)

High permeability control (90%)

Mid permeability control (50%)

Low permeability control (20%)

More Surveys